Archive List for Physical Chemistry

HPLC: Methods and Principles of Reversed-Phase Chromatography

Physical Chemistry

Chromatography is an experimental operation for the separation of substances. One of the most common experimental operations in chromatography is HPLC (high performance liquid chromatography). High performance liquid chromatography is used in almost every laboratory, including physical chemistry, organic chemistry, and biochemistry. Although the experiments performed vary from lab to lab, HPLC is the analytical […]

Mass Spectrometry: Molecular Weight and Mass Spectra in MS

Physical Chemistry

When determining the structure of a compound or detecting what compounds are present, one of the best methods is mass spectrometry (MS). When measuring a known compound, mass spectrometry can determine if it is the target compound with a high probability. Even if it is an unknown compound, mass spectrometry can be used to measure […]

Absorbance of UV-Vis Absorption Spectrum: Beer–Lambert Law

Physical Chemistry

A large number of laboratories use absorbance when analyzing in experimental manipulations. By measuring how much light passes through, you can measure how much of a substance is present in the solution. Alternatively, it can be used to check how far a reaction has progressed. When experimenting with biological systems, we frequently use absorbance to […]

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